This message should ALWAYS be in top 5

[Schleier von Dunst]

*restarts party*

[ksdfjhlksajf]

yay!!!! Party!!!

[Schleier von Dunst]

*puts on loud music*

[Tuesday]

*Does "the Robot"*

[Godsmydaddy]

GET BACK UP THERE!

[ksdfjhlksajf]

*pokes thread* is the party still going on?

*Dances anyway*

[Tuesday]

Long inverted repeats (palindromes) are ubiquitous among prokaryotic and eukaryotic genomes. Earlier work has implicated both DNA breaks and short inverted repeats (IRs) in the formation of long palindromes in yeast and Tetrahymena by a proposed mechanism of intramolecular recombination. Here we report that long-palindromic linear plasmids are formed in Streptomyces following double strand DNA breakage by a nonrecombinational intra-strand annealing process that also involves IRs. By modification of palindrome-generating linear plasmids and development of a novel procedure that enables the sequencing of palindrome junctions, we show that long-palindrome formation occurs by unimolecular intra-strand annealing of IRs followed by 3′ extension of the resulting DNA fold-back. The consequent hairpin structures serve as templates for synthesis of duplex linear plasmids containing long palindromes. We suggest that this model for long-palindrome formation in Streptomyces may represent a generally applicable mechanism for generating DNA palindromes.

[ksdfjhlksajf]

Quote:

Originally Posted by Tuesday

Long inverted repeats (palindromes) are ubiquitous among prokaryotic and eukaryotic genomes. Earlier work has implicated both DNA breaks and short inverted repeats (IRs) in the formation of long palindromes in yeast and Tetrahymena by a proposed mechanism of intramolecular recombination. Here we report that long-palindromic linear plasmids are formed in Streptomyces following double strand DNA breakage by a nonrecombinational intra-strand annealing process that also involves IRs. By modification of palindrome-generating linear plasmids and development of a novel procedure that enables the sequencing of palindrome junctions, we show that long-palindrome formation occurs by unimolecular intra-strand annealing of IRs followed by 3′ extension of the resulting DNA fold-back. The consequent hairpin structures serve as templates for synthesis of duplex linear plasmids containing long palindromes. We suggest that this model for long-palindrome formation in Streptomyces may represent a generally applicable mechanism for generating DNA palindromes.

I'm going to pretend like I understood that.

[Schleier von Dunst]

Quote:

Originally Posted by Tuesday

Long inverted repeats (palindromes) are ubiquitous among prokaryotic and eukaryotic genomes. Earlier work has implicated both DNA breaks and short inverted repeats (IRs) in the formation of long palindromes in yeast and Tetrahymena by a proposed mechanism of intramolecular recombination. Here we report that long-palindromic linear plasmids are formed in Streptomyces following double strand DNA breakage by a nonrecombinational intra-strand annealing process that also involves IRs. By modification of palindrome-generating linear plasmids and development of a novel procedure that enables the sequencing of palindrome junctions, we show that long-palindrome formation occurs by unimolecular intra-strand annealing of IRs followed by 3′ extension of the resulting DNA fold-back. The consequent hairpin structures serve as templates for synthesis of duplex linear plasmids containing long palindromes. We suggest that this model for long-palindrome formation in Streptomyces may represent a generally applicable mechanism for generating DNA palindromes.

I'm going to pretend I read more than the first line and a half.

Quote:

Originally Posted by Fairytales Tell Tales

I'm going to pretend like I understood that.

lol

[Flesh.]

Bumpeh

[Schleier von Dunst]

hepmub

[Maiku Neko-Kun]

Oh look, it isn't in the top 5

[Schleier von Dunst]

it is now.

[frantheviera]

bumperage

[Schleier von Dunst]

Ecdysis is the molting of the cuticula in arthropods and related groups (Ecdysozoa). Since the cuticula of these animals is also the skeletal support (the exoskeleton) of the body and is inelastic, it is shed during growth and a new, larger covering is formed. The old, empty exoskeleton is called an exuvia (or "exuvium").
After molting, an arthropod is described as teneral; it is "fresh", pale and soft-bodied. Within one or two hours, the cuticle hardens and darkens following a tanning process similar to that of the tanning of leather. It is during this short phase that the animal grows, since growth is otherwise constrained by the rigidity of the exoskeleton.
Ecdysis may also enable damaged tissue and missing limbs to be regenerated or substantially re-formed, although this may only be complete over a series of molts, the stump being a little larger with each molt until it is of normal, or near normal size again.

[frantheviera]

argh *bumps again*

[~IntoxicatedRainbow]

Should go back to top 5!

[ksdfjhlksajf]

*makes thread float back to the top 5*

[Flesh.]

Bumpeh

[Maiku Neko-Kun]

"This message should ALWAYS be in top 5"
And yet it isn't!! =O