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-   -   This message should ALWAYS be in top 5 (https://www.recoveryourlife.com/forum/showthread.php?t=1948)

Schleier von Dunst 15-04-2009 05:31 PM

*restarts party*

ksdfjhlksajf 15-04-2009 06:56 PM

yay!!!! Party!!!

Schleier von Dunst 15-04-2009 07:21 PM

*puts on loud music*

Tuesday 15-04-2009 08:12 PM

*Does "the Robot"*

Godsmydaddy 15-04-2009 10:32 PM

GET BACK UP THERE!

ksdfjhlksajf 16-04-2009 12:15 AM

*pokes thread* is the party still going on?

*Dances anyway*

Tuesday 16-04-2009 01:07 AM

Long inverted repeats (palindromes) are ubiquitous among prokaryotic and eukaryotic genomes. Earlier work has implicated both DNA breaks and short inverted repeats (IRs) in the formation of long palindromes in yeast and Tetrahymena by a proposed mechanism of intramolecular recombination. Here we report that long-palindromic linear plasmids are formed in Streptomyces following double strand DNA breakage by a nonrecombinational intra-strand annealing process that also involves IRs. By modification of palindrome-generating linear plasmids and development of a novel procedure that enables the sequencing of palindrome junctions, we show that long-palindrome formation occurs by unimolecular intra-strand annealing of IRs followed by 3′ extension of the resulting DNA fold-back. The consequent hairpin structures serve as templates for synthesis of duplex linear plasmids containing long palindromes. We suggest that this model for long-palindrome formation in Streptomyces may represent a generally applicable mechanism for generating DNA palindromes.

ksdfjhlksajf 16-04-2009 03:06 AM

Quote:

Originally Posted by Tuesday (Post 1555213)
Long inverted repeats (palindromes) are ubiquitous among prokaryotic and eukaryotic genomes. Earlier work has implicated both DNA breaks and short inverted repeats (IRs) in the formation of long palindromes in yeast and Tetrahymena by a proposed mechanism of intramolecular recombination. Here we report that long-palindromic linear plasmids are formed in Streptomyces following double strand DNA breakage by a nonrecombinational intra-strand annealing process that also involves IRs. By modification of palindrome-generating linear plasmids and development of a novel procedure that enables the sequencing of palindrome junctions, we show that long-palindrome formation occurs by unimolecular intra-strand annealing of IRs followed by 3′ extension of the resulting DNA fold-back. The consequent hairpin structures serve as templates for synthesis of duplex linear plasmids containing long palindromes. We suggest that this model for long-palindrome formation in Streptomyces may represent a generally applicable mechanism for generating DNA palindromes.

I'm going to pretend like I understood that. :hehe:

Schleier von Dunst 16-04-2009 11:14 AM

Quote:

Originally Posted by Tuesday (Post 1555213)
Long inverted repeats (palindromes) are ubiquitous among prokaryotic and eukaryotic genomes. Earlier work has implicated both DNA breaks and short inverted repeats (IRs) in the formation of long palindromes in yeast and Tetrahymena by a proposed mechanism of intramolecular recombination. Here we report that long-palindromic linear plasmids are formed in Streptomyces following double strand DNA breakage by a nonrecombinational intra-strand annealing process that also involves IRs. By modification of palindrome-generating linear plasmids and development of a novel procedure that enables the sequencing of palindrome junctions, we show that long-palindrome formation occurs by unimolecular intra-strand annealing of IRs followed by 3′ extension of the resulting DNA fold-back. The consequent hairpin structures serve as templates for synthesis of duplex linear plasmids containing long palindromes. We suggest that this model for long-palindrome formation in Streptomyces may represent a generally applicable mechanism for generating DNA palindromes.

I'm going to pretend I read more than the first line and a half.

Quote:

Originally Posted by Fairytales Tell Tales (Post 1555392)
I'm going to pretend like I understood that. :hehe:

lol

Flesh. 16-04-2009 12:34 PM

Bumpeh

Schleier von Dunst 16-04-2009 02:08 PM

hepmub

Maiku Neko-Kun 16-04-2009 04:15 PM

Oh look, it isn't in the top 5

Schleier von Dunst 16-04-2009 04:21 PM

it is now.

frantheviera 16-04-2009 04:26 PM

bumperage

Schleier von Dunst 16-04-2009 04:52 PM

Ecdysis is the molting of the cuticula in arthropods and related groups (Ecdysozoa). Since the cuticula of these animals is also the skeletal support (the exoskeleton) of the body and is inelastic, it is shed during growth and a new, larger covering is formed. The old, empty exoskeleton is called an exuvia (or "exuvium").
After molting, an arthropod is described as teneral; it is "fresh", pale and soft-bodied. Within one or two hours, the cuticle hardens and darkens following a tanning process similar to that of the tanning of leather. It is during this short phase that the animal grows, since growth is otherwise constrained by the rigidity of the exoskeleton.
Ecdysis may also enable damaged tissue and missing limbs to be regenerated or substantially re-formed, although this may only be complete over a series of molts, the stump being a little larger with each molt until it is of normal, or near normal size again.

frantheviera 16-04-2009 04:52 PM

argh *bumps again*

~IntoxicatedRainbow 16-04-2009 05:02 PM

Should go back to top 5!

ksdfjhlksajf 16-04-2009 06:53 PM

*makes thread float back to the top 5*

Flesh. 16-04-2009 07:04 PM

Bumpeh

Maiku Neko-Kun 16-04-2009 08:45 PM

"This message should ALWAYS be in top 5"
And yet it isn't!! =O


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